Resumo A hipóxia é uma característica proeminente do câncer de cabeça e pescoço. No entanto, as características do elemento oxigênio das proteínas e como elas se adaptam aos microambientes de hipóxia do câncer de cabeça e pescoço ainda são desconhecidas. Sequências do genoma humano e dados expressos de proteínas de câncer de cabeça e pescoço foram recuperados do atlas de patologia do projeto Human Protein Atlas. Em seguida, comparou o conteúdo do elemento de oxigênio e carbono entre proteomas de câncer de cabeça e pescoço, e células epiteliais escamosas da mucosa oral normal, localizações do genoma, vias e dissecção funcional associada ao câncer de cabeça e pescoço também foram estudadas. Um total de 902 proteínas expressas diferencialmente foi observado onde o conteúdo médio de oxigênio é maior do que as proteínas expressas de forma humilde em proteínas de câncer de cabeça e pescoço. Além disso, o conteúdo médio de oxigênio das proteínas reguladas positivamente foi 2,54% maior do que das outras. Nenhum de seus genes codificadores foi distribuído no cromossomo Y. As proteínas reguladas positivamente foram enriquecidas em endocitose, apoptose e regulação do citoesqueleto de actina. O conteúdo aumentado de oxigênio das proteínas altamente expressas e reguladas pode ser causado pela atividade frequente do citoesqueleto e adaptado ao rápido crescimento e divisão das células cancerosas de cabeça e pescoço. O viés do uso de oxigênio e as proteínas-chave podem nos ajudar a entender os mecanismos por trás do câncer de cabeça e pescoço na terapia direcionada, o que estabelece uma base para a aplicação da estequioproteômica na terapia direcionada e oferece uma promessa para potenciais tratamentos para o câncer de cabeça e pescoço.

Abstract Hypoxia is a prominent feature of head and neck cancer. However, the oxygen element characteristics of proteins and how they adapt to hypoxia microenvironments of head and neck cancer are still unknown. Human genome sequences and proteins expressed data of head and neck cancer were retrieved from pathology atlas of Human Protein Atlas project. Then compared the oxygen and carbon element contents between proteomes of head and neck cancer and normal oral mucosa-squamous epithelial cells, genome locations, pathways, and functional dissection associated with head and neck cancer were also studied. A total of 902 differentially expressed proteins were observed where the average oxygen content is higher than that of the lowly expressed proteins in head and neck cancer proteins. Further, the average oxygen content of the up regulated proteins was 2.54% higher than other. None of their coding genes were distributed on the Y chromosome. The up regulated proteins were enriched in endocytosis, apoptosis and regulation of actin cytoskeleton. The increased oxygen contents of the highly expressed and the up regulated proteins might be caused by frequent activity of cytoskeleton and adapted to the rapid growth and fast division of the head and neck cancer cells. The oxygen usage bias and key proteins may help us to understand the mechanisms behind head and neck cancer in targeted therapy, which lays a foundation for the application of stoichioproteomics in targeted therapy and provides promise for potential treatments for head and neck cancer.

ABSTRACT Abdominal cocoon syndrome is a rare cause of intestinal obstruction, which is difficult to diag- nose preoperatively. We here report a case of abdominal cocoon. A 47-year-old male patient was referred to the general surgery department with complaints of abdominal pain, distension, nausea and vomiting for 1 day. An abdominal computed tomography examination detected the dilated small intestinal loops clustered in the abdomen and surrounded by a sac-like membrane. During the exploratory surgery, a capsular structure was identified in the lower quadrant with a regular surface that was solid fibrous in nature. The combination of physical examination, imaging signs and medical history may be helpful in the diagnosis.

The effect of training system on the content and composition of flavan-3-ols in seeds throughout the development of Cabernet Sauvignon grapes during two consecutive seasons (2011 to 2012) in the northwest of China was evaluated. The applied training systems include the modified vertical shoot positioned (M-VSP); the fan training system with two trunks (F-TT); and the fan training system with multiple trunks (F-MT). The results show that the pattern of flavan-3-ol accumulation was similar for the three training systems in both vintages, with flavan-3-ols being the highest around véraison, before declining as harvest approaches. Compared to the 2011 vintage, the dry vintage of 2012 had a greater accumulation of total flavan-3-ols and individual flavan-3-ol monomers, including (+)-catechin (C), (-)-epicatechin (EC) and (-)-epicatechin-3-0-gallate (ECG). With regard to the effect of training systems, M-VSP contributed to a significantly higher content of total flavan-3-ols, while the percentage of ECG was lower for M-VSP. As for the three flavan-3-ol units (terminal subunits, extension subunits and monomers), the 2012 vintage was more likely to accumulate extension subunits and monomers, and the effect of training system on these three flavan-3-ols units varied between the different vintages. Meanwhile, the proportion of ECG in terminal subunits and of C in extension subunits was higher in 2012, whereas the training system of F-TT had a higher percentage of ECG in extension subunits in both vintages. In addition, the effect of vintage on the composition of flavan-3-ols in harvested grape seeds was more obvious than that of the training system.

The synergistic influences of three unsaturated fatty acids (UFAs, namely linoleic acid, oleic acid and linolenic acid) on the fatty acid composition of Saccharomyces cerevisiae and major volatile compounds were investigated in synthetic grape juice. The addition of UFAs led to a corresponding increase in UFAs in the cellular lipid, which was accompanied by a reverse reduction in the content of medium-chain saturated fatty acids (C6:0 to C14:0) and little variation in that of long-chain saturated fatty acids (C16:0 to C24:0). The supplementation of UFAs considerably improved yeast growth and fermentation activity and, in particular, increased the concentrations of most volatile compounds in wine, including higher alcohols (2-phenylethanol, 2-methyl-1-propanol and 3-(methylthio)-1-propanol), medium-chain fatty acids (butanoic acid, hexanoic acid and octanoic acid), acetate esters (isoamyl acetate and 2-phenylethyl acetate) and all ethyl esters. Remarkable linear relationships were further found between ethyl esters and the concentration of the added UFAs (R² from 0.909 to 0.996), which significantly intensified the fruity, flowery and sweet attributes of the final wine, as assessed by calculating the odour activity values. Our results suggest that rationally increasing the concentration of UFAs is not only a practical method to improve yeast fermentation activity, but also a potential approach to manipulating wine aroma.

'Zaoheibao' (a red tetraploid hybrid) and 'Wuhecuibao' (a white triploid hybrid) grapes have been obtained from Guibao ♀ (diploid, Vitis vinifera) x Zaomeigui ♂ (diploid, V. vinifera) and Guibao ♀ (diploid, V. vinifera) x Wuhebaijixin♂ (triploid, V. vinifera) respectively. Aroma characterisation of the two new table grape cultivars was firstly done by the investigation of volatile compounds. The influence of greenhouse cultivation and vintage on berry aroma was studied as well. The results showed that linalool, decanal, β-damascenone, hexanal and (E)-2-hexenal were the main volatile compounds of the two cultivars, which meant that the floral, fruity and sweet odour were prominent, followed by the herbaceous aroma. Greenhouse cultivation enhanced herbaceous odour in both 'Wuhecuibao' and 'Zaoheibao' berries, and reduced the floral aroma, contributed mainly by β-damascenone, in 'Wuhecuibao', and the sweet aroma, represented mainly by linalool, in 'Zaoheibao'. The concentrations of the main aroma compounds were greatly affected by vintage and the intensity of sensorial perception was correspondingly changed, but varietal odour attributes were not significantly altered. These results will not only help promote the cultivation and popularisation of these cultivars, but also will provide valuable data for the use of these cultivars in future breeding.

Proanthocyanidins in grape berries are synthesised mainly before véraison, and very little attention is paid to the evolution of proanthocyanidins (PAs) in grapes from véraison to harvest. The present study focused on the changes of flavan-3-ols with different degrees of polymerisation in grape skins and the difference in proanthocyandin composition of 'Shiraz', 'Cabernet Sauvignon' and 'Marselan' grapes (Vitis vinifera L.). The results show that the content of flavan-3-ols, the percentage of prodelphinidins (%P) and mean degree polymerisation (mDP) found in 'Cabernet Sauvignon' berry skins at post-véraison were higher than those in 'Shiraz' and 'Marselan' skins. Only monomelic, dimeric, trimeric and polymeric flavan-3-ols were detected in the three grape cultivars. Polymers with more than tenfold flavan-3-ol units accounted for a relatively high proportion in grape berry skins, and the content in the three cultivars declined continuously during ripening. Principal component analysis showed that proanthocyanidin content, composition and mDP at grape harvest stage depended strongly on grape cultivar. This study provides some useful information for understanding the accumulation of PAs during berry maturation and this information can be used to improve wine quality.

A 9-year field micro-plot experiment was conducted to investigate the use efficiency, residual effect, and fate of 15N-labelled ryegrass green manure in soil-crop system, so as to offer useful information for reasonable application of green manures on meadow burozem. Results showed that high application rate (600g per plot) of 15N-labelled ryegrass green manure resulted in reduction of percent recovery (PR)15Nplant and PR15Nsoil by 25.0% and 12.8%, respectively, and elevation of percent loss (PL)15N by 31.2%, as compared with low application rate. The 15N utilization and loss could be divided into three phases, i.e., high uptake and fast loss in the first and second growth seasons, low uptake and slow loss in the following four growth seasons, and little change in the last three growth seasons. This suggested that the green manure N had a long residual effect but its loss was faster at early phase, and thus, rational application of green manure was of considerable importance to reduce its N loss while improve its N use efficiency.

The degradation of cephalexin in aqueous solution under ultrasound irradiation was investigated. Biodegradability of the solution was evaluated by the BOD5/COD ratio, which was raised from zero to 0.36 after ultrasound treatment, indicating that the ultrasound irradiation process is a successful pre-treatment step to improve the biodegradability of cephalexin solution. The influences of ultrasound power and pH value on the degradation of cephalexin were studied. It was found that the optimal ultrasound power for cephalexin degradation in the system was 200 W and the rate of cephalexin degradation was maximal in the pH range of 6.5 to 8.5. The degradation kinetics of cephalexin in aqueous solution under various operational conditions was also investigated. It was found that the degradation of cephalexin follows a pseudo-first order kinetics.

The seismic moment cycle at plate boundaries may be imaged by the Moment-Ratio Method (MRM). In this exploratory technique the fluctuation of cumulative moment deficiency is used as an indicator of the cycle of degradation and recovery of free energy along an active tectonic region. A 20-year half-life is assumed for the healing process of earthquake ruptures along the Pacific plate boundary. Using the Pacheco-Sykes Catalog of large shallow earthquakes (M>7), we estimate the moment ratio for Japan and Mexico at 20-year intervals during the 20th century. Moment-ratio peaks for 1980 correlate with subsequent earthquakes of magnitude M>7 during the following 20-year period in Mexico and Japan.

El Método de Momentos Residuales (MRM) puede servir para monitorear en forma gráfica el ciclo del momento sísmico en un borde de placa. Es una técnica exploratoria que permite observar las deficiencias del valor acumulado de los momentos sísmicos para evaluar el ciclo de degradación y recuperación de la energía libre en una región sísmica activa. Se adopta una vida media de 20 años en el proceso de cicatrización de las rupturas sísmicas en el borde de la Placa del Pacífico. Se estima el momento residual para las regiones de Japón y México a intervalos de veinte años con el Catálogo de Pacheco y Sykes de sismos someros de magnitud M>7 en el siglo 20. Los picos de MRM tienden a mostrar alguna correlación con sismos de magnitud mayor que 7 que ocurrieron en el intervalo siguiente de 20 años.

High mobility group box 1 (HMGB1) was discovered as a novel late-acting cytokine that contributes to acute lung injury (ALI). However, the contribution of HMGB1 to two-hit-induced ALI has not been investigated. To examine the participation of HMGB1 in the pathogenesis of ALI caused by the two-hit hypothesis, endotoxin was injected intratracheally in a hemorrhagic shock-primed ALI mouse model. Concentrations of HMGB1 in the lung of the shock group were markedly increased at 16 h (1.63 ± 0.05, compared to the control group: 1.02 ± 0.03; P < 0.05), with the highest concentration being observed at 24 h. In the sham/lipopolysaccharide group, lung HMGB1 concentrations were found to be markedly increased at 24 h (1.98 ± 0.08, compared to the control group: 1.07 ± 0.03; P < 0.05). Administration of lipopolysaccharide to the hemorrhagic shock group resulted in a notable HMGB1 increase by 4 h, with a further increase by 16 h. Intratracheal lipopolysaccharide injection after hemorrhagic shock resulted in the highest lung leak at 16 h (2.68 ± 0.08, compared to the control group: 1.05 ± 0.04; P < 0.05). Compared to the hemorrhagic shock/lipopolysaccharide mice, blockade of HMGB1 at the same time as lipopolysaccharide injection prevented significantly pulmonary tumor necrosis factor-alpha, interleukin-1beta and myeloperoxidase. Lung leak was also markedly reduced at 16 h; blockade of HMGB1 24 h after lipopolysaccharide injection failed to alter lung leak or myeloperoxidase at 48 h. Our observations suggest that HMGB1 plays a key role as a late mediator when lipopolysaccharide is injected after hemorrhagic shock-primed ALI and the kinetics of its release differs from that of one-hit ALI. The therapeutic window to suppress HMGB1 activity should not be delayed to 24 h after the disease onset.